| |
Level |
K’ |
T |
(Rs) |
| A: pH of mobile phase |
| 2.8 |
-0.2 |
4.8 |
1.43 |
2.4 |
| 3 |
0 |
4.5 |
1.43 |
2.4 |
| 3.2 |
0.2 |
4.5 |
1.45 |
2.2 |
| Mean ± S.D (n=6) |
4.5 ± 0.3 |
1.43 ± 0.020 |
2.3 ± 0.1 |
| B: Flow rate (mLmin-1) |
| 0.8 |
-0.2 |
4.1 |
1.45 |
2.2 |
| 1 |
0 |
4.3 |
1.44 |
2.6 |
| 1.2 |
0.2 |
4.4 |
1.44 |
2.7 |
| Mean ± S.D (n=6) |
4.3 ± 0.212 |
1.44 ± 0.015 |
2.36 ± 0.026 |
| C: Percentage of water in mobile phase (v/v) |
| 25 |
-5 |
4.6 |
1.42 |
2.38 |
| 30 |
0 |
4.3 |
1.43 |
2.36 |
| 35 |
5 |
4.5 |
1.46 |
2.33 |
| Mean ± S.D (n=6) |
4.36 ± 0.070 |
2.36 ± 0.025 |
2.36 ± 0.025 |
| C: Wavelength (nm) |
| 225 |
-5 |
4.5 |
1.42 |
2.38 |
| 235 |
0 |
4.3 |
1.43 |
2.36 |
| 240 |
5 |
4.4 |
1.45 |
2.32 |
| Mean ± S.D (n=6) |
4.3 ± 0.070 |
1.43 ± 0.015 |
2.36 ± 0.030 |
K’=Capacity factors, N=Theoretical plates, T=Tailing factor, Rs=Resolution |
