Parameters LC method UV method VIS method
Useful concentration, μg/mL 6 6 6
Concentration range, μg/mL 5.0-15.0 7.0-12.0 8.0-13.0
Analytical curve 44299X + 137924 0.0868X -0.0425 0.0255X – 0.0257
Determination coefficient (r2) 0.9990 0.9995 0.9970
R.S.D. of repeatability (%) 0.08 0.83 0.73
R.S.D. intermediate precision (%) 1.23 2.22 0.88
Accuracy (%) 98.14 98.1 98.63
RSD of accuracy (%) 0.74 0.9 0.68
LOQ, µg/mL 2.89 0.73 3.82
LOD, µg/mL 0.95 0.24 1.26
Table 1: Validation parameters for chromatographic, visible and UV quantitation methods for azumolene.
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