**Figure 3: **Pareto charts showing the standardized effect of factors, X_{1} flow rate: mL/min, X_{2} injection volume: µL, X_{3} detection wavelength: nm, X_{4} initial gradient acetonitrile ratio: % v/v, X_{5} acetonitrile ratio at four minutes of gradient run: % v/v, in Plackett-Burman (PB) design for the responses: (A) Y_{1} peak area of STP: mAU.s, (B) Y_{2} peak area of HI443: mAU.s, (C) Y_{3} USP Tailing of STP, (D) Y_{4} USP Tailing of HI443. Bars extending past the vertical lines indicates the values reaching statistical significance (α=0.05). Asterisk (*) indicates the significant effect (p<0.05). (E) Prediction profiler and desirability plot in PB design. |