Figure 5: Defect rate analysis of the factors, X1 flow rate: mL/min, X2 injection volume: µL, X3 initial gradient acetonitrile ratio: % v/v, for the responses, Y1 peak area of STP: mAU.s, Y2 peak area of HI443: mAU.s, Y3USP Tailing of STP, and Y4 USP Tailing of HI443, shown by: (A) Defect rate profiler. (B) Defect parametric profiler. (C) Gaussian process plot. (D) Marginal model plots, (E) Prediction profiler plot for Log10 defect rate and desirability plot.
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