Variables Levels
  Low Medium High
Independent variables (factors)      
X1; Flow rate: mL/min 0.6 0.8 1.0
X2; Injection volume: ┬ÁL 10 15 20
X3; Detection wavelength: nm 265 270 275
X4; Initial gradient acetonitrile ratio: % v/v 60 70 80
X5; Acetonitrile ratio at four min of gradient run: % v/v 85 90 95
Coded levels of levels of X1, X2, X3, X4 and X5 -1 0 +1
Dependent variables (responses)
Y1; Peak area of STP: mAU.s
Y2; Peak area of HI443: mAU.s
Y3; USP Tailing of STP
Y4; USP Tailing of HI443
Table 1:Variables with their corresponding values in the experimental design.
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