Sample Concentration spiked (mg/L) Concentration found (mg/L) (Mean ± SD) Bias (%) Imprecision CV (%)
Flucloxacillin        
  LLOQ 0.200 0.208 ± 0.0092 3.92 4.4
  QC1 2.00 2.14 ± 0.083 6.83 3.9
  QC2 20.0 20.8 ± 1.48 4.00 7.1
  QC3 100 102 ± 7.95 2.43 7.8
Cefazolin    
  LLOQ 0.200 0.201 ± 0.0065 0.33 3.3
  QC1 2.00 1.99 ± 0.112 -0.33 5.6
  QC2 20.0 18.7 ± 0.34 -6.42 1.8
  QC3 100 93.9 ± 3.49 -6.15 3.7
Table 2: Inter-day assay variance of the determination of flucloxacillin and cefazolin in plasma (n = 6).
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