Parameter Values
BCG CR MO
pH 3.0 5.0 2.5
Extracting solvent chloroform Chloroform chloroform
λmax 420 515 480
Molar ratio (SUL : Dye) 1 : 1 1 : 1 1 : 1
Beer’s law limits (μg/mL) 2 – 14 2 – 16 2 -14
Molar absorptivity (L mol-1cm-1) 4.1×104 2.1×104 3.5×104
Sandell’s sensitivity (ng cm-2) 10.2 26.3 12.3
Range of error % 0.74 : -0.60 0.51 : 0.24 0.86 : -0.91
Regression equation*      
Intercept 0.236 ± 0.0018 0.184 ± 0.0022 0.016 ± 0.002
Slope 0.098 ± 0.003 0.038 ± 0.002 0.081 ± 0.003
Correlation coefficient (r) 0.9990 0.9991 0.9998
Limit of quantification (μg/mL) 0.15 0.30 0.20
Limit of detection (μg/mL) 0.044 0.095 0.064
pkf 5.50 4.25 4.80
*A=a+bC, where C is the concentration in μg/mL
Table 1: Quantitative parameters for spectrophotometric determination of SUL as ion-pair complexes with BCG, CR, and MO.
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