| Peptide |
Q1 Precursor Ion |
Precusor m/z Ratio |
Q3 Product Ion |
Optimized Collision Energy (V) |
Retention Time (min) |
| QVLQVTPFAER |
644.4 |
+2 |
1060.4 (y9)* |
33.4 |
9.5 |
| QVLQVTPFAER |
644.4 |
+2 |
819.5 (y7) |
33.4 |
9.5 |
| QVLQVTPFAER |
644.4 |
+2 |
720.5 (y6) |
33.4 |
9.5 |
| GEGIEEFLR |
525.3 |
+2 |
863.5 (y7) |
28.1 |
10.3 |
| GEGIEEFLR |
525.3 |
+2 |
693.4 (y5)* |
28.1 |
10.3 |
| GEGIEEFLR |
525.3 |
+2 |
564.5 (y4) |
28.1 |
10.3 |
|
| *denotes most intense fragment ion for the VTG peptide biomarker candidate |
| Table 3: Optimized Fragmentation (Product) Ions for the two X. laevisVitellogenin Peptide Biomarkers. |
