Analyte (n=6 each matrix) Matrix LLOQ Area(mean) Blank Area (mean) Result (%) Acceptance criteria Propofol Plasma 4422.2 311 7.03 <20% Liver 6637.3 290.3 4.40 Kidney 3171.8 299 9.42 Lung 4462.8 487.7 10.93 Heart 3900.8 182.2 4.70 2,6-diisopropyl-1,4-quinol Plasma 734.5 16.2 2.20 <20% Liver 1664.3 17.3 1.04 Kidney 2103 nd - Lung 2436.2 22.7 0.93 Heart 1845.2 14.5 0.79 2,6-diisopropyl-quinone Plasma 1363.7 41 3.0 <20% Liver 4807.2 32.7 0.67 Kidney 3650 nd - Lung 3792.5 31.7 0.83 Heart 3284 12 0.36 IS (Tymol) Plasma 109169 683.3 0.62 <5% Liver 119696 874.2 0.73 Kidney 114529 928.8 0.81 Lung 121898 490.5 0.40 Heart 115054 753.7 0.66

IS – internal standard; LLOQ – Lower limit of quantification; nd – not detected

Table 1: Selectivity validation of propofol, 2,6-diisopropyl-1,4-quinone and 2,6-diisopropyl-1,4-quinol in different matrices (plasma, liver, kidney, lung and heart).

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