Analyte
(n=6 each matrix)
Matrix
LLOQ Area
(mean)
Blank Area
(mean)
Result
(%)
Acceptance criteria
Propofol
Plasma
4422.2
311
7.03
<20%
Liver
6637.3
290.3
4.40
Kidney
3171.8
299
9.42
Lung
4462.8
487.7
10.93
Heart
3900.8
182.2
4.70
2,6-diisopropyl-1,4-quinol
Plasma
734.5
16.2
2.20
<20%
Liver
1664.3
17.3
1.04
Kidney
2103
nd
-
Lung
2436.2
22.7
0.93
Heart
1845.2
14.5
0.79
2,6-diisopropyl-quinone
Plasma
1363.7
41
3.0
<20%
Liver
4807.2
32.7
0.67
Kidney
3650
nd
-
Lung
3792.5
31.7
0.83
Heart
3284
12
0.36
IS (Tymol)
Plasma
109169
683.3
0.62
<5%
Liver
119696
874.2
0.73
Kidney
114529
928.8
0.81
Lung
121898
490.5
0.40
Heart
115054
753.7
0.66
IS – internal standard; LLOQ – Lower limit of quantification; nd – not detected
Table 1:
Selectivity validation of propofol, 2,6-diisopropyl-1,4-quinone and 2,6-diisopropyl-1,4-quinol in different matrices (plasma, liver, kidney, lung and heart).
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