Analyte
(n=6 each matrix)
Matrix LLOQ Area(mean) Blank Area
(mean)
Result
(%)
Acceptance criteria
Propofol Plasma 4422.2 311 7.03 <20%
Liver 6637.3 290.3 4.40
Kidney 3171.8 299 9.42
Lung 4462.8 487.7 10.93
Heart 3900.8 182.2 4.70
2,6-diisopropyl-1,4-quinol Plasma 734.5 16.2 2.20 <20%
Liver 1664.3 17.3 1.04
Kidney 2103 nd -
Lung 2436.2 22.7 0.93
Heart 1845.2 14.5 0.79
2,6-diisopropyl-quinone Plasma 1363.7 41 3.0 <20%
Liver 4807.2 32.7 0.67
Kidney 3650 nd -
Lung 3792.5 31.7 0.83
Heart 3284 12 0.36
IS (Tymol) Plasma 109169 683.3 0.62 <5%
Liver 119696 874.2 0.73
Kidney 114529 928.8 0.81
Lung 121898 490.5 0.40
Heart 115054 753.7 0.66
IS – internal standard; LLOQ – Lower limit of quantification; nd – not detected
Table 1: Selectivity validation of propofol, 2,6-diisopropyl-1,4-quinone and 2,6-diisopropyl-1,4-quinol in different matrices (plasma, liver, kidney, lung and heart).