Analyte
(n=6 for each) |
Carry-over |
Matrix effects |
LLOQ Area
(Mean) |
Blank Area (Mean) |
Result
(%) |
Acceptance criteria |
CV (%) |
Acceptance criteria |
| LC |
HC |
| Propofol |
4518.98 |
314.04 |
6.95 |
<20% |
6.46 |
7.12 |
<15% |
| 2,6-diisopropyl-quinol |
1756.64 |
14.14 |
0.80 |
<20% |
8.32 |
5.43 |
<15% |
| 2,6-diisopropyl-quinone |
3379.48 |
23.48 |
0.69 |
<20% |
10.22 |
7.43 |
<15% |
| IS |
116069.2 |
746.1 |
0.64 |
<5% |
4.82 |
5.19 |
<15% |
|
| IS – internal standard; LLOQ – Lower limit of quantification; LC – lower concentration; HC – High concentration |
| Table 2: Carry-over and matrix effects validation of propofol, 2,6-diisopropyl-1,4-quinone and 2,6-diisopropyl-1,4-quinol. |
