Figure 5: Representative MRM chromatograms of (a) blank fat sample obtained from a patient during TKA surgery, (b) supernatant of immersed fat tissue suspensions spiked with vancomycin at 10 mg/L, (c) fat sample obtained from a patient after intraosseous injection of vancomycin during TKA surgery (vancomycin concentration = 4.9 mg/L).