Sample Concentration
spiked (mg/L)
Concentration found (mg/L)
(Mean ± SD)
Bias
(%)
Imprecision
CV (%)
Intra-day        
  LLOQ 0.050 0.046 ± 0.0044 -8.00 9.6
  QC1 1.00 1.02 ± 0.027 2.67 2.2
  QC2 10.0 9.55 ± 0.23 -4.51 2.4
  QC3 50.0 51.7 ± 1.31 3.45 2.53
Inter-day    
  LLOQ 0.050 0.051 ± 0.0053 6.25 10.0
  QC1 1.00 1.05 ± 0.062 5.87 5.9
  QC2 10.0 10.0 ± 0.32 0.45 3.2
  QC3 50.0 52.5 ± 1.10 5.0 2.1
Table 3: Intra- and inter-day assay variance of the determination of vancomycin in supernatant of immersed fat tissue suspension (n = 6).
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