Figure 2: Pareto chart showing the effect of the independent variables (X1, volume of protein solution; X2, volume of TCA solution), on the absorbance of the supernatant recorded at 280 nm and the percentage of protein precipitated using BCA assay. Sorted parameter estimates and their corresponding t-ratio are shown on the horizontal-axis. Bars extending beyond the vertical line indicate values reaching statistical significance (α = 0.05).