Independent variables |
Level |
Coded values |
-1.414 |
-1 |
0 |
+1 |
+1.414 |
X1 = Volume of protein 2 mg/mL solution (µL) |
880.6 |
980.0 |
1220.0 |
1460.0 |
1559.4 |
X2 = Volume of 6.1 N TCA solution (µL) |
11.7 |
20 |
40 |
60 |
68.3 |
Dependent variables |
|
|
|
Y1= Absorbance of the supernatant recorded at 280 nm |
Y2 = Percentage of protein precipitated (% w/w) |
|