Lack-of-fit test analysis to check

Response	Source	DF^a	SS^b	MS^c	F-ratio^d
Y₁	Lack-of-fit	3	0.167	0.055	5857.55
	Pure error	4	0.000	0.023	Prob> F
	Total error	7	0.167		<0.0001
Y₂	Lack-of-fit	3	29.240	9.745	0.964
	Pure error	4	40.450	10.112	Prob>F
	Total error	7	69.687		0.491

^adegree of freedom

^bSum of Square

^cMean sum of square

^dModel MS/error MS

Table 4: Lack-of-fit test analysis to check the model adequacy predicting the absorbance of the supernatant (Y₁), and the percentage of protein precipitated (Y₂).

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