Concentration (ng/mL) Statistical parameters
Actual conc. Calculated con. Mean SD % CV Accuracy
set-1 set-2 set-3
31.26 31.48 31.19 31.13 31.27 0.187 0.6 100.03
62.51 62.33 64.05 62.38 62.92 0.979 1.6 100.65
208.37 196.10 191.59 213.18 200.29 11.389 5.7 96.12
744.19 792.70 771.43 771.45 778.53 12.274 1.6 104.61
2126.25 2178.08 2165.30 2185.57 2176.32 10.249 0.5 102.35
4725.00 4953.16 5031.85 4869.14 4951.38 81.370 1.6 104.79
7560.00 7736.82 7657.28 7808.41 7734.17 75.600 1.0 102.30
9000.00 8261.12 8364.61 7866.51 8164.08 262.847 3.2 90.71
10000.00 9755.35 9960.23 9813.10 9842.89 105.639 1.1 98.43
Table 1: Calculated concentrations of cholic acid calibration standards prepared in rat plasma (n=3).