Storage condition Analyte concn.
(ng/mL)
Calculated con.(ng/mL) % CV Accuracy (%)
Plasma Urine Plasma Urine Plasma Urine
Three freeze-thaw cycles 100.80 105.96 ± 3.091 94.66 ± 4.737 2.9 5.0 105.1 93.9
8400.00 7902.81 ± 34.742 8059.43 ± 180.754 0.4 2.2 94.1 95.9
Autosampler
 (24 hr)
100.80 106.28 ± 4.775 107.68 ± 2.734 4.5 2.5 105.4 106.8
8400.00 7878.79 ± 168.324 7851.06 ± 285.283 2.1 3.6 93.8 93.5
Bench top
(6 hr)
100.80 104.81 ± 2.272 100.65 ± 5.470 2.2 5.4 104.0 99.9
8400.00 8037.10 ± 133.286 8057.51 ± 259.936 1.7 3.2 95.7 95.9
Table 5: Summary of stability studies for cholic acid in plasma and urine.