| |
L-AA QC/STD expected concentration (µg/mL) |
Ret time (min) |
COV |
Measured concentration (µg/mL) |
COV |
REC4 (%) |
| |
|
|
|
|
|
|
| Intermediate precision1 |
10 (L-AA STD) |
3.038 ± 0.02 |
0.54 |
9.14 ± 2.46 |
2.46 |
|
| |
50 (L-AA STD) |
3.041 ± 0.01 |
0.30 |
48.61 ± 2.81 |
2.81 |
|
| |
125 (L-AA STD) |
3.042 ± 0.01 |
0.46 |
123.18 ± 1.96 |
1.96 |
|
| Intra-assay precision2 |
6.5 (QC) |
3.023 ± 0.004 |
0.12 |
6.59 ± 1.644 |
0.38 |
101.41 ± 0.40 |
| |
55 (QC) |
3.023 ± 0.003 |
0.11 |
56.76 ± 6.780 |
0.19 |
103.19 ± 0.19 |
| |
115 (QC) |
3.022 ± 0.003 |
0.09 |
114.05 ± 8.568 |
0.12 |
99.18 ± 0.14 |
| Injection precision3 |
55 (QC) |
3.031 ± 0.02 |
0.52 |
54.11 ± 0.49 |
0.49 |
|
|
| 1Intermediate precision values are means ± SD of three determinations (n=3) assayed over three separate days. 2Intra-assay precision values are means ± SD of three
determinations (n=3) assayed on the same day. 3Injection precision values are means ± SD of six determinations (n=6). 4Spiked recovery method used for accuracy.
Abbreviations: COV: Coefficient of Variation; L-AA STD, L-ascorbic acid Standard; QC: Quality Control; REC: Recovery |
| Table 2: Precision of the UV-HPLC method for the determination of L-ascorbic acid. |
