| Samples/standards/QC |
Changes |
Retention time |
Response |
| pH 2.81 |
COV |
REC (%) |
COV4 |
| Camu powder |
|
0.075 |
102.13 |
2.42 |
| ;Tropical juice |
|
0.094 |
98.94 |
0.62 |
| Mix berry juice |
|
0.635 |
96.83 |
1.90 |
| Pressed orange juice |
|
0.099 |
102.48 |
1.42 |
| Distilled water/acetonitrile/formic acid
(80.9: 19: 0.1, v/v/v)2 |
| QC (60 µg/mL) |
|
0.33 |
99.92 |
0.73 |
| Camu powder |
|
0.12 |
103.56a |
2.03 |
| Onion |
|
4.64 |
99.47 |
3.89 |
| Column temperature: 20°C3 |
| Camu powder |
|
0.33 |
102.61 |
1.51 |
| 10 µg/mL L-AA standard |
|
0.27 |
100.25 |
1.09 |
| 20 µg/mL L-AA standard |
|
0.20 |
98.93 |
2.64 |
| 50 µg/mL L-AA standard |
|
0.29 |
99.44 |
1.88 |
| 100 µg/mL L-AA standard |
|
0.01 |
97.16 |
3.08 |
| Column temperature: 26°C |
| 5 µg/mL L-AA standard |
|
0.29 |
101.31 |
2.30 |
| 20 µg/mL L-AA standard |
|
0.78 |
97.59 |
2.97 |
| 50 µg/mL L-AA standard |
|
2.13 |
97.83 |
2.56 |
| 100 µg/mL L-AA standard |
|
0.05 |
96.06 |
3.48 |
| 125 µg/mL L-AA standard |
|
0.07 |
97.86 |
1.34 |
| QC sample (60µg/mL) |
|
0.18 |
99.83 |
0.72 |
| camu powder |
|
0.26 |
101.71 |
1.00 |
|
1Optimized HPLC method at pH 2.6. 2Mobile phase composition for optimized method: distilled water/acetonitrile/formic acid (99: 0.9: 0.1, v/v/v). 3Column temperature of
optimized method: 23°C.
4Precision of assay performed at optimal conditions and with variations. aSignificantly (p<0.05) higher recovery obtained with modified mobile
phase |
| Table 4: Evaluation of the robustness of the HPLC method for L-AA determination. |
