| |
|
Retention time (min)1 |
PAA (AU) |
Height (AU) |
Capacity factor (K´) |
Theoretical plates (N) |
Resolution (Rs) |
Peak asymmetry factor (As) |
Selectivity factor (ȴ) |
| Day |
1 |
3.035 |
257.86 |
55.09 |
7.62 |
10561 |
7.91 |
0.89 |
1.45 |
| |
2 |
3.034 |
263.41 |
55.27 |
7.47 |
10749 |
7.53 |
0.86 |
1.45 |
| |
3 |
3.035 |
255.99 |
55.20 |
7.48 |
10850 |
7.89 |
0.92 |
1.45 |
| |
4 |
3.034 |
253.12 |
55.21 |
9.18 |
10747 |
7.91 |
0.88 |
1.46 |
| |
5 |
3.035 |
258.70 |
55.88 |
7.36 |
10752 |
7.7 |
0.9 |
1.46 |
| Mean ± SD |
|
3.035 ± 0.001 |
257.8 ± 3.8 |
55.3 ± 0.3 |
7.482 ± 0.092 |
10731.8 ± 104.9 |
7.788 ± 0.169 |
0.890 ± 0.022 |
1.454 ± 0.005 |
| COV |
|
0.018 |
1.470 |
0.568 |
1.234 |
0.978 |
2.172 |
2.512 |
0.377 |
|
| 1The retention time for the unresolved peak is 0.357 ± 1.33 SD. Abbreviations: AU, absorbance unit |
| Table 5: System suitability testing for the HPLC assay for the determination of L-AA in food and beverage products. |
