Statistic parameters Serum Urine
Depeptization by 6% HClO4 SPE SPE
0.025
[mg/ml]
0.05
[mg/ml]
0.1
[mg/ml]
0.025
[mg/ml]
0.05
[mg/ml]
0.1
[mg/ml]
0.05
[mg/ml]
0.075
[mg/ml]
0.1
[mg/ml]
Mean amount fund (mg/ml) 0.0197 0.045 0.091 0.0187 0.037 0.062 0.032 0.048 0.083
Standard deviation (s) 0.0039 0.007 0.014 0.0011 0.0024 0.0016 0.0022 0.0023 0.0077
Variance (S2) 1.5·10-5 4.9·10-5 2·10-4 1.2·10-6 5.8·10-6 2.6·10-6 4.8·10-6 5.3·10-6 5.9·10-5
Relative standard deviation 0.1969 0.1554 0.1595 0.0599 0.0652 0.0256 0.0674 0.0474 0.0923
Coefficient of variation [%] 19.69 15.54 15.95 5.99 6.52 2.56 6.74 4.74 9.23
95% confidence interval 8.35·10-5 3.12·10-4 3.12·10-4 2.41·10-5 5.22·10-5 3.41·10-5 4.70·10-5 4.94·10-5 1.66·10-4
Spread 0.009 0.016 0.037 0.003 0.006 0.005 0.006 0.007 0.019
Relative error [%] 21.2 10 1 25.2 26 38 36 36 17
Recovery [%] 78.67 89.33 90.67 74.53 74.2 61.63 66.33 64.33 83.57
Table 1: Precision and accuracy assay results for investigated compound on spiked serum and urine samples analyzing using different pretreatment methods.