| Oligonucleotide |
Sequence (5’? 3’) |
Size |
| Capture probe |
GAGTAAAGTTAATACCTTTGCTCATTGACG |
30-mer |
| Target Probe |
CGTCAATGAGCAAAGGTATTAACTTTACTC |
30-mer |
| Forward primer (63F) |
CAGGCCTAACACATGCAAGTC |
21-mer |
| Reverse primer (1389R) |
ACGGGCGGTGTGTACAAG |
18-mer |
| E.coli 16S r-DNA (amplified sequence) |
- |
1447 bp |
| Target fragment of E.coli 16S rDNA (Acil, restriction enzyme product) |
GGTAACGTCAATGAGCAAAGGTA TTAACTTTA CTCCCTTCCTCC |
44-bp |
