Figure 2:An example of the Northern blot detection of peptidyl-tRNA enzymatic hydrolysis and its inhibition. The probe used was [32P]-tRNALys. In Lane 1, peptidyl-tRNA migration on the gel results in a characteristic “smear” due to heterogeneity of the peptide constituent. In Lane 2, a single band corresponding to tRNALys is a result of enzyme hydrolysis. In Lane 3, Pth inhibition prevents peptidyl-tRNA hydrolysis and the observed peptidyl-tRNA is identical to that in Lane 1.