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| Figure 2: Performance of the three-day ELISA. (A) Plot of the absorbance signal against VLP concentration using a broad range of VLP concentrations (0.037 to 3.7 μg/mL) and 0.0005 mg/mL HRP-labeled secondary antibody; (B) Absorbance values and the linear range obtained in using VLP concentration ranging between 0.037 to 0.925 μg/mL; and (C) Absorbance values and the linear range obtained using the three-hour ELISA for detection of GI.1 VLPs in the same concentration range as the 3-day ELISA. Two different concentration levels (0.0001 and 0.0002 mg/mL) of HRP-label secondary antibody were used in (B) and (C). The concentration of primary mAb 3901 anti-VLP antibody used in all assays was the same (0.0002 mg/mL). |
