Figure 5: Performance of the “signal-down” capture ELISA. (A) Absorbance values for the detection of GI.1 VLPs in the range of 0 to 3.7 μg/mL, using two different concentrations of the capture antibody (0.0001 and 0.0002 mg/mL mAb 3901 anti-VLP antibody). (B) Linear regression between the absorbance signals and the logarithmic values of GI.1 VLP concentrations ranging from 0.037 to 2.04 μg/mL. Both assays used 0.0001 mg/mL HRP-conjugated goat anti-mouse IgG antibody.
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