Method R2 Value Linear  Range (μg/mL) Detection Limita   (μg/mL)
Three-Day (3-d) ELISA 0.95-1.0 0.037-0.555 0.153 (L1)b; 0.015 (L2)c
Three-Hour (3-h) ELISA 0.98-0.998 0.037-0.555 0.164 (L1); 0.046 (L2)
Three-Hour (3-h) Fluorescent Immunoassay 0.9985 0.5-2.0 0.313 (L2)
“Signal-Down” Capture ELISA 0.94-0.99d 0.037-2.040 0.00058 (L1); 14.4 (L2)
aDetection limit was calculated using LOD = XBlank +3 σ with 99% confidence
level.
bL1 denotes the lower primary (3-d, 3-h ELISA) or secondary (3-h immunoassay)
antibody employed in the assay.
cL2 denotes the higher primary (3-d, 3-h ELISA) or secondary (3-immunoassay)
antibody employed in the assay.
dR2 values were the coefficient between the absorbance signal and the logarithmic
value of VLP concentration.
Table 1: Correlation Values, Ranges, and Limits
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