Peak No. tR (min) UV λmax (nm) [M+H]+ (m/z) Fragment ions (+) [M-H]ˉ (m/z) Fragment ions (-) Compound
1 26.66 229,275,305(sh) NDa NDa 165 150,122,107 Apocynin
2 28.57 218,319(sh) 391 NDa 389 227,182,143 Picied
3 33.81 265,332(sh) 463 301,183,103,85 461 446,283,227,162,136 Tectoridin
4 37.59 213,263,339(sh) 463 343,329,301,286,127,109,85 461 446,283,255,239,195,167 Tectorigenin-4`-glucoside
5 38.64 215,265,339(sh) 523 361,346,310,282 521 491,359,343,313 Iridin
6 55.03 264,314 301 285,268,168,122 299 284,255,240,211,132,110 Tectorigenin
7 57.97 221,266 361 345,315,303,245,208,193,107 359 344,328,329,314,285,258,270,191 Irigenin
8 60.64 219,289,344(sh) NDa NDa 301 286,253,166,155,140,120,110,87 5,2`,3`-trihydroxy-7-methoxyflavanone
9 66.21 264,333(sh) 387 357,342,326,311,279,206, 165,132,120 NDa NDa Irisflorentin
10 67.12 224,297,348(sh) NDa NDa 313 298,224,213,192,166,138,123,110 Crocetenone
11 68.90 263,346(sh) 315 299,285,269,254,169,133 313 298,283,267,255,211,183,167,141,117 5,7-dihydroxy-6,2`-dimethoxyisoflavone
tR: Retention time.
Sh: shoulder peak.
aThe ion signals of this component investigated were not detected in this ion mode.
Table 1: Chromatorgraphic, UV and mass spectral characteristics of marker compounds.