Peak No. tR (min) UV λmax (nm) [M+H]+ (m/z) Fragment ions (+) [M-H]ˉ (m/z) Fragment ions (-) Compound
1 8.7 206,253,288 NDa NDa 329 295,255,204,186,155,108,85 Irispurinol
2 13.08 262 NDa NDa 785 469,452,344,236,204,147 Tectorigenin  4`-gentiobioside-7-glucoside
3 14.80 210,263,329(sh) 625 509,232,109 623 461,446,359,298,90 Tectoridin 4`-glucoside
4 15.40 231,264 NDa NDa 653 613,532,513,490,442,387
,363,310,254,231,182
Iristectoregenin B 4`-diglucoside
5m 29.07 263 625 621,463,356
,331,301,273
623 477,419,257,213,155 Tectorigenin 7-diglucoside
6 30.80 229,266,340(sh) 493 331,324,316
,256,215,180,168
NDa NDa Iristectorin B
7 31.74 260 433 392,352,348,304,280,
271,243,225,153,130
NDa NDa Gentenstein 7-glucoside
8m 33.26 264,329(sh) 463 301,183,103,85 461 446,283,227,162 Tectoridin
9m 37.26 217,263,339(sh) 463 343,329,301
,286,127,109,85
461 446,283,255,239,195,167 Tectorigenin 4`-glucoside
10 40.85 257 853b 678,652,610
,490,471,453
829 707,656,227,64 Iridalglycoside 5b/6c
11 41.36 260 855b 652,597,547,521,490
,454,393,309,262
830 668,601,476,315,258 Iridalglycoside 7/8
12m 54.12 265,314 301 285,281,268,
189,168,133,122
299 269,55 Tectorigenin
13 57.46 243,264,334(sh) NDa NDa 301 252,207,80 5,8,2`-trihydroxy-7-methoxyflavnone
tR: Retention time.
m: Marker compounds.
sh: shoulder peak.
aThe ion signals of this component investigated were not detected in this ion mode.
bThis fragment ion was [M+Na]+.
Table 4: Identified compounds in Iris spuria by HPLC-DAD-ESI-MS/MS.