Gene PCR primer pair  Gene sequence
(5′- 3′)
Annealing
Temp.
(°C)
PCR Product size (bp) Reference
Common
NPRS
MTF2
MTR
GCNGG(CT)GG(CT)GCNTA(CT)GTNCC
CCNCG(AGT)AT(TC)TTNAC(TC)TG
  50   1000   [79]
  mcyA MSF
MSR
ATCCAGCAGTTGAGCAAGC
TGCAGATAACTCCGCAGTTG
  50   1300   [25]
u-102F
u-620R
CGATGAACAAATCGGGCAATGGCA
TGCAAGTTTCGCACATCTCCAAGG
  55   518   This study
  mcyB P5102F
P5853R
AGTCATCATCTTCCTTCACCGCGT
TGTCCTGCCATCCGTTCAATCGTA
  55   751   This study
2156-F
3111-R
ATCACTTCAATCTAACGACT
AGTTGCTGCTGTAAGAAA
  44   955   [39]
mcyC PSCF1
PSCR1
GCAACATCCCAAGAGCAAAG
CCGACAACATCACAAAGGC
  49   674   [35]
mcyD PKDF2
PKDR2
AGTTATTCTCCTCAAGCC
CATTCGTTCCACTAAATCC
  44   859   [35]
mcyE PKEF1
PKER1
CGCAAACCCGATTTACAG
CCCCTACCATCTTCATCTTC
  48   755   [35]
mcyG PKGF1
PKGR1
ACTCTCAAGTTATCCTCCCTC
AATCGCTAAAACGCCACC
  48   425   [35]
Table 1: Suitable PCR primers used in this study for the amplification of mcy genes.
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