Table 2a | LC-ESI-MS identification and quantification..

LC signal (Figure 6)	Microcystin Congener	Retention time (min)	Major LC-ESI-MS ions (m/z)	Standard curve linearity (r2)	Amount extracted (µg/g DW)
i	MC-RR	6.61	134.7 (ADDA); 1039.3 [M+H]+	0.9963	53.620 ± 0.063
ii	MC-YR	7.50	135.4 (ADDA); 1046.2 [M+H]+; 523.3 [M+2H]²⁺	0.9948	5.280 ± 0.035
iii	MC-LR	7.77	135.8 (ADDA); 996.3 [M+H]+	0.9948	12.114 ± 0.024
iv	MC-WR	8.37	135.0 (ADDA); 1069.3 [M+H]+	n.a	*1.714 ± 0.001

Key: *MC-LR equivalent concentration; n.a: Not Applicable; MC amounts are average values of n=3

Table 2a: LC-ESI-MS identification and quantification of MCs extracted from toxigenic M. novacekii cells collected from Phakalane pond effluent. The MCs were preconcentrated using SPE Oasis WatersTM HLB cartridges after the removal of pigments and less polar compounds by a liquid partitioning step in methanolic aqueous CHCl₃.

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