LC signal
(Figure 6)
Microcystin Congener Retention time (min) Major LC-ESI-MS ions (m/z) Standard curve linearity (r2) Amount extracted
 (µg/g DW)
i MC-RR 6.61  134.7 (ADDA);
1039.3 [M+H]+
0.9963 53.620 ± 0.063
ii MC-YR 7.50  135.4 (ADDA);
1046.2 [M+H]+;
523.3 [M+2H]2+
0.9948 5.280 ± 0.035
iii MC-LR 7.77 135.8 (ADDA);
996.3 [M+H]+
0.9948 12.114 ± 0.024
iv MC-WR 8.37 135.0 (ADDA);
1069.3 [M+H]+
n.a *1.714 ± 0.001
Key: *MC-LR equivalent concentration; n.a: Not Applicable; MC amounts are average values of n=3
Table 2a: LC-ESI-MS identification and quantification of MCs extracted from toxigenic M. novacekii cells collected from Phakalane pond effluent. The MCs were preconcentrated using SPE Oasis WatersTM HLB cartridges after the removal of pigments and less polar compounds by a liquid partitioning step in methanolic aqueous CHCl3.
Goto home»