Figure 2: Levels of NLRP3 protein in TAMs co-incubated with PDT-treated and untreated SCCVII cells. Cells from untreated SCCVII tumors (including TAM population) were co-incubated for 16 hours with in vitro grown SCCVII cells (either untreated or treated by Photofrin-PDT as described for Figure 1). The cells were then collected and stained for F4/80 antigen to identify TAMs and for intracellular NLRP3 protein to assess inflammasome activity in these cells based on flow cytometry analysis. The bars denote SD; *=statistically significant difference from the value in control TAMs ; **=statistically significant difference from the value in TAMs co-incubated with untreated SCCVII cells