Figure 4: Ceramide and S1P levels in TAMs from SCCVII tumors following co-incubation with un-separated PDT-treated SCCVII cells. Cells from untreated SCCVII tumors were plated together with in vitro grown SCCVII cells treated by PDT as described for Figure 1 and incubated for 3 or 5 hours before collection for flow cytometry. Antibody staining, including anticeramide (a) or anti-S1P (b) was as described for Figure 3. The bars denote SD; *=statistically significant difference from the values in control TAMs not co-incubated with SCCVII cells.
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