Matrix Chemical Name Specificity Solubility in Water DHB 2,5-dihydroxybenzoic acid Lipids,peptides, <10KDa proteins Soluble CHCA α-cyano-4- hydroxycinnamic acid Peptides,<10KDa proteins Moderately soluble SPA 3,5-dimethoxy-4- hydroxycinnamic acid >10 KDa proteins Insoluble CMBT 5-chloro-2-mercapto- benzothiazole Glycolipids, peptides, <10KDa proteins Soluble

Table 1: Salient biochemical properties of the four matrices used in this study and their corroboration with the mass spectral data derived for the Gram positive gen- era Clostridium, Listeria and Propionibacterium.

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