Figure 2: Comparison of disaccharide elution between filters. The peak corresponding to Di-4S (DS) in the chromatogram after use of the Ultra filtration Omega 10K filter was much larger than seen for the other filters. The amount of KS1 (mono-sulfated KS) and KS2 (di-sulfated KS) (right panel) was also higher from this filter than the neonatal screening filter (note scale of y-axis). Elution of HS was similar for all 3 filters (data not shown).*AcroPrep™ Advance 96-Well Filter Plates. |