Analyte Information
Method Optimization
QC Preparation Appropriate to Range
Evaluation of Extraction Strategies (Protein Precipitation, LLE/SLE, SPE)
- Phospholipid impact evaluation
- Recovery
- Matrix Stability
- Extract Stability
- Initial Carryover Evaluation
Post-column infusion experiment
Mock Validation Run
- Accuracy and Precision
- Carryover
- Freeze / Thaw Stability
- Bench Top (pre-processed) Stability
- Long Term Stability
- Recovery
- Matrix Effect
- Selectivity
Length of run should mimic longest expected sample set, padded with blanks |
