Overview of Method Development Exercises

Analyte Information

  • Structure
  • Properties

Method Optimization
QC Preparation Appropriate to Range
Evaluation of Extraction Strategies (Protein Precipitation, LLE/SLE, SPE)

  • Phospholipid impact evaluation
  • Recovery
  • Matrix Stability
  • Extract Stability
  • Initial Carryover Evaluation

Post-column infusion experiment
Mock Validation Run

  • Accuracy and Precision
  • Carryover
  • Freeze / Thaw Stability
  • Bench Top (pre-processed) Stability
  • Long Term Stability
  • Recovery
  • Matrix Effect
  • Selectivity

Length of run should mimic longest expected sample set, padded with blanks

Table 1: Overview of Method Development Exercises.
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