Figure 5:
Effect of pH on the retention. PFPP Column: Discovery HS F5 (5μm, 2.1x50 mm), mobile phase: 5 mM ammonium formate buffer (pH 2.9, 3.1, 3.7, 4.3 and 6.4) in 80% acetonitrile, flow rate: 0.2 mL/min, column temperature: 40°C.
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