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| Figure 6: Effect of acetonitrile composition on the retention time. ODS: Luna C18 (2) (5μm, 2 x 50 mm), PFPP: Discovery HS F5 (5μm, 2.1x50 mm), mobile phase: 5mM ammonium formate buffer (pH 2.9) in 70, 80, 85, 90, 95, 99.5 % acetonitrile, flow rate: 0.2 mL/min, column temperature: 40°C. |
