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Figure 8: Peak deterioration with analyte concentrations. Sample amount applied to the PFPP column, Discovery HS F5 (5μm, 2.1x50 mm) : 0.00002 nmol to 5.66 nmol diluted with a mobile phase, 5mM ammonium formate buffer (pH 2.9) in 80 % acetonitrile; flow rate, 0.2 mL/min; column temperature, 40°C.
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