Validation sample Bias Within-run precision (n=3) Between-run precision (n=3) 2 pmol/106 cells 11.7% 11.0% Negligible 20 pmol/106 cells 4.5% 6.2% Negligible 200 pmol/106 cells −6.4% 16.2% Negligible Negligible, smaller value than the Within-run precision

Table 1: Bias and Precision of Individual Validation Sample Concentrations for Ara- GTP in Cell Extracts.

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