Matrix
Compound
Concentration(ng/mL)
Extracted spiked steroid in matrix (ng/mL)
De-conjugated steroid concentration (ng/mL)
Plasma
Testosterone
1
0.98
0.91
2
2.08
2.03
15
16.96
14.03
Epitestosterone
1
1.09
1.15
2
2.11
2.14
15
16.18
14.85
Urine
Testosterone
1
1.02
1.09
2
2.04
2.07
15
15.81
15.29
Epitestosterone
1
1.08
0.96
2
1.97
1.94
15
15.87
15.06
Table 5:
The concentration of testosterone and epitestosterone after de-conjugation by β-glucuronidase compared to standard concentrations of extracted testosterone and epitestosterone in plasma matrix.
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