Figure 3: Tissue specific distribution of free LCBs in rice. (A) Structure of NBD-F and its derivatives of LCBs. (B) Principal component analysis of free LCB composition in various rice tissues.To obtain principal components (PCs), LCB contents were converted to Z scores and analyzed using R program (ver. 3.0.2, prcomp2 package) [38]. Scatter plot of the first two components PC1 (indicating 35.8% of variance) vs. PC2 (indicating 29.8% of variance) clearly showed tissuespecific distribution of free LCBs. (C,D) Contents of free forms of total and saturated species (C) and unsaturated ones (D) in rice tissues. Data are mean ± SD (n=4).
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