SSP # Ca/ Chelator MW Ca/Chelator *Fold change Protein expression change Ca Protein expression change BAPTA/ EGTA Detected by LC/MS ID Gene name Protein class 7816/6807 63.6/87.4 * 2.11; 2.41 ↓10mMCa ↑Bapta 0.5mM 45Ca Probable Cation transport ATPase 92% coverage copA Class I 6514/7503 42.27/53.51 * 2.49; 4.94 ↓5mMCa ↓10mMCa ↑0.25mM Bapta 45Ca Anti-CaM Ab Flagellin 99% coverage hag Class I 8205/7205 28.37/32.01 * 5.69; 8.41 ↓5mMCa ↑EGTA 1mM 45Ca Adenylate kinase 99% coverage adk Class I 6312/6401 35.05/47.58 * 2.41; 2.4 ↓10mMCa ↑0.25Bapta ↑1mM EGTA 45Ca Fructose- biphosphate aldolase 99% coverage fbaA Class I 8202/8501 30.19/52.32 *5.69; 5.69 ↓10mMCa ↑0.25mM Bapta ↑0.5mM Bapta 45Ca EF-Hand Ab Heat-shock protein 99% coverage grpE Class I 9102/9103 19.99/23.52 *2.5; 2.5 ↓5mMCa ↑0.25 Bapta ↑0.5mM Bapta 45Ca Hypothetical protein BS12280 99% coverage rex Class I

Table 3: Characteristics of differentially expressed proteins identified by 45Ca- 760 autoradiography, induced by Ca2+- chelators and reduced by high CaCl2 761 concentration.

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