Step
Time
Flow rate (ml/min)
Mobile Phase A
Mobile Phase B
0
0.00
0.300
50
50
1
1.00
0.300
90
10
2
2.00
0.300
90
10
3
4.00
0.300
50
50
4
5.00
0.300
50
50
Table 1a:
Gradient elution program of chromatographic separation.
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