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| Figure 1: 2D-PAGE pattern of the cashew stem proteins extracted by the Phenol-Tris based method. Proteins (300 μg) were separated by IEF on a 3-10 linear pH gradient strip (13 cm) followed by SDS-PAGE and visualized after staining with colloidal CBB. Numbered protein spots were excised from the gel and identified using nano-UPLC-ESI-Q-TOF analysis. The identified proteins are listed in Table 1 and Table S1 (supplementary material). |
