1 Carbon 2 [Carbon] 3 [Substr] 4 Substr. add. time 5 = 123 Shak. 6 = 134 Lum. 7 = 234 Temp. 8 = 1234 Extr. time
f1 -1 -1 -1 -1 -1 -1 -1 1
f2 1 -1 -1 -1 1 1 -1 -1
f3 -1 1 -1 -1 1 -1 1 -1
f4 1 1 -1 -1 -1 1 1 1
f5 -1 -1 1 -1 1 1 1 -1
f6 1 -1 1 -1 -1 -1 1 1
f7 -1 1 1 -1 -1 1 -1 1
f8 1 1 1 -1 1 -1 -1 -1
f9 -1 -1 -1 1 -1 1 1 -1
f10 1 -1 -1 1 1 -1 1 1
f11 -1 1 -1 1 1 1 -1 1
f12 1 1 -1 1 -1 -1 -1 -1
f13 -1 -1 1 1 1 -1 -1 1
f14 1 -1 1 1 -1 1 -1 -1
f15 -1 1 1 1 -1 -1 1 -1
f16 1 1 1 1 1 1 1 1
Levels (-1 and +1) description
-1 Soluble starch 10 g.L-1 5 mg 1 day Static Dark 25°C 12 days
1 Dextrose 50 g.L-1 40 mg 7 days 200 rpm Light 35°C 20 days
Table 1: 28-4 fractional factorial design for biotransformation of naringin by M. inaequisporus with factors and its levels (-1 and 1) in different flasks (f1-f16).
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