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| Figure 1: (A) SPR sensorgrams related to detection of PB1-F2 with a chip-immobilized specific anti-PB1-F2 antibody. After purification recombinant PB1-F2 was diluted in 10 mM sodium acetate buffer, pH5 and run over the chip surface, (10-1500 nM). Then the buffer was run over to remove unbound protein. RU, arbitrary resonance unit. (B) Calibration plots showing the change of immunosensor responses as a function of different concentrations of PB1-F2 (■), and unrelated control proteins, Shadoo (●) or N-terminal part of prion protein (★). |
