| Methods |
DIRECT METHOD
Geometry of the test: conjugation between IgG, immobilized on the membrane of immunosensor and the excess of urease conjugated anti-IgG, free in solution.
(a) |
COMPETITION METHOD
Geometry of the test: competition between IgG, immobilized on the membrane of immunosensor, and IgG free, for the urease conjugated anti-IgG, free in solution
(b) |
Regression equation
(Y= a.u., X= M)
Confidence level (1-α) = 0.95; |
Y = -19.4 (± 3.1) × -77.9 (± 13.4)
(n–v) = 6 ; (t=2.45) |
Y = -41.3 (± 4.3) log × -323.9 (± 18.4)
(n–v) = 9 ; (t=2.26) |
| Linear range (M) |
2×10-5 - 7×10-8 |
6.0×10-8 ÷ 3×10-10 |
| Correlation coefficient |
0.9813 |
0.9801 |
Repeatability of the
measurement
(as pooled SD%) |
6.8 |
8.6 |
Low detection limit
(LOD) (M) |
4×10-8 |
1.7×10-10 |
| Instrumental response time (minutes) |
@ 10 |
@ 15 |
(a.u.=mV), Employed transducer: potentiometric electrode for NH