Table 1 | Comparison of calculation methods for..

Calculation Method	Lineweaver-Burk	Hofstee
Abs_max	0.0254	0.0249
Michaelis Menten constant (K_M)	0.313 mM	0.301 mM
Formula	1/v = -12.385 1/[S] + 39.45	[S] / v = 40.109 [S] -12.083
Abs_max calculation	Abs_max = 1/c Abs_max = 1/39.45 Abs_max = 0.0254	Abs_max = 1/m Abs_max = 1/40.19 Abs_max = 0.0249
K_M calculation	K_M = m × Abs_max K_M = 12.385 × 0.0254 K_M = 0.313 mM	K_M = c × Abs_max K_M = 12.03 × 0.0249 K_M = 0.301 mM

Table 1: Comparison of calculation methods for K_M and Abs_max based on Lineweaver-Burk and Hofstee equations.