**Calculation Method** |
**Lineweaver-Burk** |
**Hofstee** |

Abs_{max} |
0.0254 |
0.0249 |

Michaelis Menten constant (K_{M}) |
0.313 mM |
0.301 mM |

Formula |
1/v = -12.385 1/[S] + 39.45 |
[S] / v = 40.109 [S] -12.083 |

Abs_{max} calculation |
Abs_{max} = 1/c
Abs_{max} = 1/39.45
Abs_{max} = 0.0254 |
Abs_{max} = 1/m
Abs_{max} = 1/40.19
Abs_{max} = 0.0249 |

K_{M} calculation |
K_{M} = m × Abs_{max}
K_{M} = 12.385 × 0.0254
K_{M} = 0.313 mM |
K_{M} = c × Abs_{max}
K_{M} = 12.03 × 0.0249
K_{M} = 0.301 mM |