Symbol Definition Value inlet analyte concentration 1-1000 pM mobile gA monomers 109 molecules/cm2 tethered binding sites 1010 molecules/cm2 gA dimers 109 molecules/cm2 tethered gA monomers 1010 molecules/cm2 W, X ,Y, Z initial concentration 0 molecules/cm2 forward reaction rate 4×106 M-1s-1 forward reaction rate 5-9 cm2s-1 molecules-1 forward reaction rate 10-10 cm2s-1 molecules-1 reverse reaction rate 10-6s-1 reverse reaction rate 10-6 s-1 reverse reaction rate 1.5×10-2 s-1 DA Diffusivity of analyte A 10-6 cm2/s LW Width of flow chamber 3.0 mm L Length of flow chamber 0.7 mm h Height of flow chamber 100 µm Q Flow Rate 100 µL/min

Table 1: Model Parameters for ICS Detection of Streptavidin.

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