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Figure 2: Electropherograms of BSA samples labeled on-column with a 25.0 mM citric acid buffer (pH 3.4) containing 5.00 x 10-6 M SQHN-3c: Concentrations of injected BSA samples as follows: (a) 10.0 x 10-6 M; (b) 5.00 x 10-6 M; (c) 2.50 x 10-6 M; (d) 1.25 x 10-6 M; (e) 6.25 x 10-7 M. Electropherograms offset for clarity. Other separation conditions:25 μm ID x 30.0 cm total length x 25.4 cm effective length capillary; pressure injection at 4 psi*sec; 20 kV separation voltage. |